565 11 2804 2 10138 document 2804 Appendix File S2.xlsx application/vnd.openxmlformats-officedocument.spreadsheetml.sheet 3f997201f46fe6b53f441d60e1b365dc MD5 41709 2026-07-13 12:31:29 https://bordar.bournemouth.ac.uk/565/1/Appendix%20File%20S2.xlsx 565 1 1 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet spreadsheet Comparative genomic and epidemiological data for plasmids related to pESBL-PH-2018 en public cc_by_nd_4

Appendix File S2.xlsx

full_archive archive 12277 disk0/00/00/05/65 2026-07-31 15:31:13 2026-07-31 15:31:24 2026-07-31 15:31:13 data_collection show 1 Fordham Stephen i7643648@bournemouth.ac.uk pub Klebsiella pneumoniae; plasmid genomics; antimicrobial resistance; comparative genomics; horizontal gene transfer. Comparative genomic and epidemiological data for plasmids related to pESBL-PH-2018. The file contains data for 61 plasmids identified as highly similar to the outbreak-associated multidrug-resistance plasmid pESBL-PH-2018. It includes plasmid and host-strain identifiers, bacterial species and sequence types, countries and dates of isolation, clinical or environmental sample sources, and sequence-similarity measurements derived from Mash and BLAST analyses. The file also reports plasmid lengths, recombination-filtered core single-nucleotide polymorphism distances, SNP densities relative to pESBL-PH-2018, and selected antimicrobial-resistance, virulence and stress-associated determinants detected in the bacterial host strains. These data support the assessment of the plasmid’s structural conservation, geographic and temporal distribution, host range and potential horizontal dissemination. 2025-04-25 published MDPI https://www.mdpi.com/2036-7481/16/5/90 10.18746/bmth.data.00000565 Comparative genomics / plasmid epidemiology Stephen Mark Edward Fordham, Anna Mantzouratou and Elizabeth Sheridan bordar@bournemouth.ac.uk Fordham Stephen i7643648@bournemouth.ac.uk Pfizer Global Medical Grants 68198087 Data were collected through an in silico comparison of the outbreak-associated plasmid pESBL-PH-2018 against 93,823 complete plasmid sequences downloaded from the NCBI RefSeq plasmid database on 16 July 2024. Mash was used to identify closely related plasmids, retaining sequences with ≥0.98 similarity and a shared-hash fraction of ≥0.90; this produced a dataset of 61 related plasmids. Plasmid size, bacterial host, sequence type, isolation source, country and collection date were retrieved from the corresponding NCBI records. Sequence similarity and coverage were assessed using BLASTn, while core single-nucleotide polymorphisms were identified using Snippy and recalculated after removal of predicted recombinant regions with Gubbins. The resulting genomic similarity measurements, epidemiological metadata and host-associated genomic characteristics were compiled in Appendix File S2. 2012 2023