This dataset encompasses detailed kinematic measures derived from fluoroscopic imaging of cervical vertebrae during controlled motion tasks. Key Features: Data Formats: The database is available in its raw form as a MATLAB (.mat) file and processed form in Excel spreadsheets, providing flexibility for various analytical needs. Comprehensive Data: It includes participant pseudonyms, participant demographics, and effective radiation dose information. Kinematic Measures: Detailed cervical spine kinematic measures, including vertebral body positions, vertebral angles relative to the image x-axis, intervertebral translation, and intervertebral disc height, are calculated using established methods (Frobin et al., 2002a; Frobin et al., 2002b). High-Resolution Imaging: The data is derived from 1024x1024 16-bit images captured at 15 frames per second during lateral view fluoroscopic recordings. Data Collection Protocol: Participants were positioned using a motorised guided motion frame, ensuring precise alignment and motion constraints. Fluoroscopic images were acquired during flexion and extension movements, with vertebral body positions tracked and analysed using bespoke image tracking techniques. Anonymised and Structured Data: The database ensures participant anonymity and provides structured data with clear identifiers for each imaging event. The MATLAB file includes subfields for participant demographics, vertebral body positions, and fluoroscopic imaging doses. The Excel files present calculated kinematic measures synchronised at 15 samples per second. Research Applications: This dataset is valuable for studies on cervical spine motion, spinal manipulation effects, and biomechanical modelling. Researchers can explore vertebral and intervertebral motion, contributing to a deeper understanding of cervical spine mechanics. For detailed methods, refer to Branney, Jonathan, Breen, Alexander, du Rose, Alister, Mowlem, Philip, and Breen, Alan (2024). Disc degeneration and cervical spine intervertebral motion: a cross-sectional study in patients with neck pain and matched healthy controls